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tions about thirt\- micra thick on the Minot micro- tome, the latter method being more satisfactory be- cause the brain substance is more easily and com- pletely dissolved. The paraffin sections were first subjected to xylol to dissolve the paraffin, then ■washed with absolute alcohol, ether, and water, and •dissolved in a fifteen per cent, antiformin solution, which was heated to 60° C, for more rapid dis- solving. The solution obtained was almost clear, with the exception of a few particles. This was then filtered through sterile gauze into sterile cen- trifuge tubes and centrifuged with from 2,500 to 3,000 revolutions. The scanty sediment was again dissolved in a fresh antiformin solution, centri- fuged, and the remaining sediment washed with sterile water and centrifuged for at least one hour. Smears were made from the sediment on cover glasses, which were cleaned with sulphuric and chromic acid. The air dried smears were fixed in absolute alcohol for fifteen minutes and stained by Gram and counter stained with carbol fuchsin. The microscopical examination of these smears showed Gram stained, Buy Generic Viagra Overnight nonacid, resistant microor- ganisms in form of bacilli, one half to one micron in length and one third micron thick, and either is- olated or arranged in chains or groups. These ba- cilliform microorganisms were similar to those de- scribed previously in the smear of fixed virus, except that the latter were finer and paler Gram stained. Besides these small bacilli, there were larger, oval, or round forms, which were arranged in Buy Generic Viagra Overnight pair^. chains, or groups. Also a granulated bacilliform was found. It was remarkable that often a larger form was found on one end of the chain anrl the succeeding ones gradually decreased in size to a very minute form. I was able Buy Generic Viagra Overnight to find these organisms in the brain of twelve dogs infected with street virus. In some cases they were present in such numbers as to be found in every Buy Generic Viagra Overnight field. In others the}- were few in number and difficult to find. These organisms could be found in only three out of twelve rabbits infected with fixed virus. As a control, the Buy Generic Viagra Overnight normal brain of the dog, rab- bit, guineapig, and human being were used. Also, pathologically changed brains of human beings were used, btit in none of these were these organisms found. The question arises whether these microorgan- isms should be considered as the cause of hydro- phobia. At present we can say that they are constantly found in the brain of dogs infected with street virus. In the fixed virus they were found in only a few cases, which probably can be accounted for b>' lower resistance to outer invasion. By the tise Buy Generic Viagra Overnight of Buy Generic Viagra Overnight a fifteen to twenty per cent, antiformin solution, which destroys all micro- organisms except tubercle bacilli, Spirochceta lym- phatica, and Bacterium lymphaticum, we may con- sider this organism to bear a certain relation to hy- drophobia. Also, the morphological properties of this microorganism, which varies from distinct mi- croscopical size to very minute forms, Buy Generic Viagra Overnight favor their aitiological connection. The definite solution of the specificity of these organisms must be confirmed Buy Generic Viagra Overnight by culture and animal experiments. References. 1. Negri. Zeitsclirift fiir Hygiene mid Jtifcktions- krankheiten, Ixiii, 1909. 2. Babes. Atlas der patlwlogischen Histologic dc< Scrvcnsystem, vii, Tafel IV. 1896. Zeitsclirift fiir Hy- giene. 1907, Ivi. 3. J. Koch and Riesling. Zeitsclirift fiir Hygiene und InfektioHskrankhciten, l.xv, p. 85, 1910. J. Koch. II. Mitteihing. Zeitsclirift fiir Hygiene una Infcktionskraiikheiten, Lwi, p. 443, 1910. 4. Lentz. Zeitsclirift fiir Hygiene und Infektions- krankheiten, Ixi. 5 P. Remlinger. Comptes rendus de la Societe de Bi- ologic. Ivi, p. 150, 1904. 6. Bertarelli. Centralblatt fiir Bakteriologie, xxxix. p. 408, 1905. 7. A. M. Stimson. Facts and Problems of Rabies, Hygienic Laboratory Bulletin No. 65, June, 1910. 8. Kolle and Wassermann. Handbuch der pathogenen Microorganisiinen, Marx, Lyssaimmunitat. p. 1273. CLINIC.\L IMPORTANCE OF THE IMPURE FIRST SOUND AT THE CARDIAC APEX. By Selian Neuhof, M. D., New York. Mechanically speaking the heart is a hollow mus- cular pump whose muscle layers are intricately in- terlaced and whose individual muscle elements are differently constituted from the rest of the striated muscle fibres of the body. .Analogous to the differ- ence in power of skeletal muscles, we sometimes find a small heart possessing great muscular power and heavier and bulkier (though normal) hearts weak and flabby (i). This difference of power NEUHOf: IMPURE FIRST APICAL SOUND. 785