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is, without doubt, of a nuclear origin, and it is pos- sible that the Buy Viagra Online Overnight Negri bodies in street virus may be of the same origin. I wish to reserve for a later communication the discussion of the interpretation against the parasitic nature of the Negri body. i greater importance is the finding of cocci and bacilli form org:anisnis by Babes and Koch. Babes found these with a modified Cajal-Giemsa stain, Koch with Heidenheim hjematoxylon stain, and later with von Krogh staining method on bichloride fixed material. I can confirm these findings, Buy Viagra Online Overnight but it is difficult to differentiate these form elements from similar ones which can be found normally in the central nervr^us system. The nature of these form elements must at pres- ent be questioned, as their isolation and cultivation have not been accomplished. Our incomplete knowledge of the finer histologi- cal details of the central nervous system, little knowledge of the pathological changes, the unac- countable influence of our fixation methods, and the difficulty in obtaining constant results in staining make it almost impossible to dififerentiate the un- known microorganisms from the cellular elements. Systematic investigations in our laboratory, by examination of smears and sections made from the central nervous system of animals naturally Buy Viagra Online Overnight in- fected with hydrophobia (dogs, cats, horses) and of rabbits inoculated with fixed virus, showed a pe- culiar form of microorganism in only one specimen. The smear was made from the brain of a rabbit infected with fixed virus and killed in the paralytic stage. The smear was freshly fixed with bichloride- alcohol for twenty-four hours, stained with Gram, and counter stained with carbol fuchsin. Yer)- small cocci and bacilliform organisms were found, which were mostly in clumps and stained light blue. The bacilli were bipolar stained with a very deli- cate, merely visible body. Without doubt, I dealt here with a peculiar kind of microorganism, as in- fection with other microorganisms is excluded, since bacteriological examination shows aerobic and anaerobic cultures sterile. The examination of the stained smear with polarized light showed no double refraction, which excludes the possibility of a precipitate resembling microorganisms. Further, after being preserved for one year and being al- most decolorized, the smear was restained and showed the same picture. All future efforts to find this same microorganism were useless, regardless of different fixations and staining methods. The remarkable resistancy of the street virus to outer invasion led me to think of the possibility of isolating Buy Viagra Online Overnight the microorganism after the destruction of the brain substance. At present, what do we know of the nature of the virus of rabies? We know that in a pure culture the virus is abundantly present in the central nervous system. Filtration experiments have shown that the rabies virus does not pass through filters which are im- permeable Buy Viagra Online Overnight to other bacteria. According to Rem- linger (5), it should pass Buy Viagra Online Overnight the larger pores of a Berkcfeld filter No. 5, which is impermeable to the smallest present known microorganism. Bertarel- lie (6) and Volpino state that the fixed virus passes through finer filters than the street virus, but that the filtration experiments arc not always trustworthy. We must bear in mind the possibility of an imper- fect filter. Stimson (y) reported that he never succeeded in obtaining a virulent filtrate of fixed virus. My own experiments have alwavs been with negative results. The filtrate which Rertarellie and Volpino ob- tained showed certain biological changes in com- 784 NEUHOF: IMPURE FIRST APICAL SOUND. JOL parison to the original material, thus increasing the time of incubation from nine to thirteen days, de- creasing the virulence, Buy Viagra Online Overnight and lowering the resistancy to chemical and physical influences. Of special in- terest is the fact that the filtrates do not contain the entire amount of the infectious agent, as part remains on the filter, which is more virulent than the original material (decreasing Buy Viagra Online Overnight time of incuba- tion, Bertarellij. According to these experiments, we have to admit that certain form elements in the rabies virus must Buy Viagra Online Overnight be microscopically visible. The investigations of the conditions of the resistance of the microbe of the rabies shows that it belongs to the class of highly resistant viruses. Putrefaction does not seem to destroy its virulence. G. E. Mattei (8) re- ports that he found no diminution in the activity of the organism in a dog which had been buried eight months. Buy Viagra Online Overnight The street virus is still more resistant to the bactericidal power of the organism, which lives for a long time in the cicatrix in Buy Viagra Online Overnight contrast to the fixed virus which is rapidly destroyed. Basing on these properties of the .street virus, first, that it must be microscopically visible, since it is only partially filterable, second, the resistancy to putrefaction, I tried to isolate the imknown mi- crobe by dissolving" in antiformin, the brain of ani- mals infected with street virus. For these experiments we use the brain of dogs, which have been proved by the inoculation of rab- bits to be afflicted with hydrophobia. The brain was hardened in formalin and ctit with the frozen microtome or embedded in paraffin and cut in sec-