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0.2 c.c in test tube 2 0.5 c.c in test tube 3 i.o c.c in test tube 4 2.0 c.c in test tube 5 A portion of the urine was then boiled, cooled, Viagra Buy Generic and added to the tubes as follows: 1.0 Viagra Buy Generic c.c in test tube i 0.8 Viagra Buy Generic c.c in test tube 2 0.5 c.c in test tube 3 Nothing in test tube 4 Nothing in test tube Viagra Buy Generic 5 The tube= were then corked and put in an incu- bator (38° C.) for six hours, and that tube which was clear at the end of this time was taken as the limit of digestion, and the amount of pepsin cal- culated in the total quantity of urine. Wilenko states that this method is easv of ap- plication and gives fairly accurate results. The salts which are apt to interfere with pepsin diges- tion, namely, urates and Viagra Buy Generic sulphates, Viagra Buy Generic are rendered nil by hydrochloric acid and by the length of time in the incubator. The ricin solution should be made up fresh every two or three days according to W ilcnko, but this time consuming work may be avoided, with no diminution of accuracy, by keep- ing the ricin in the cold. Viagra Buy Generic Diastase: In each cf seven tubes are measured 5 c.c. of a one per cent, rice starch solution. This starch solution is made by stirring one gramme of soluble starch CKahlhaum') into 100 c.c. of water. stirring until a uniform suspension results. This is put in a porcelain dish on the water bath, and under constant stirring is kept there until clear, which occurs in from eight to ten minutes. This solution lasts for several days, but fresh solutions are advo- cated. In the first tube Wohlgemuth puts i c.c. of the urine, in the next 0.64, in the next 0.4, in the next 0.25, in the next 0.16, in the next o.i, and in the next 0.064. To each tube is added two drops of toluol, and the tubes are put in an incubator at 38° C. tem- perature and allowed to remain there for twenty-four hours. Thev are then taken out, rapidly cooled by surrounding them with ice, and the tubes are filled almost to the top with water and then one drop of a i/io normal iodine solution is added to each tube. That tube which first shows a blue tint is considered the limit of activity and the strength of the diastase is calculated from the tube just below this one. He describes a factor D2 .j^, which is supposed to repre- sent the amount of diastase in i c.c. of solution. For instance, if 0.16 c.c. showed blue (limes) and 0.25 showed no trace of blue, then D^.j^ equals 20. I have placed in one column Wohlgemuth's factor D^.u and in another column Viagra Buy Generic the total amount of diastase TD-^ , . This is arrived at bv assuming go 24h that D2.JJ represents the amount in 100 c.c. and then multiplying this result by the number of times too goes into the total quantity of urine. In this wav the amount of diastase in the twenty-four hour (juantity can be Viagra Buy Generic estimated. If D^.jj equals 20 and if there were 1,600 c.c. voided in twenty-four hours, there would be 320 diastase units present. It seems more advisable to make an arbitrary calculation such ,g° as this instead of multiplying D^,]^ (the amount in I c.c.) by 1,600, which would give 32,000 diastase units. The same idea of amount can be had by dis- regarding the last two ciphers, in other words by ^go Viagra Buy Generic assuming that D^/ih represents the amount in 100 c.c. of urine. The total quantity of diastase is then ,go represented in the last column by the factor TD^^j^ All twenty-four hour specimens regardless of the diseases were examined, our object being to test out in a large series the practical advantage to be de- rived from a study of the ferments. The cases have been grouped as nearly consequentially as possible and when two or more conditions are coexistent, the primary or principal morbid state has been chosen for tabulation of the case. CLINICAL WORK. The work of Leo (/. c), Bendersky (/. c.), Mya and Belfanti (26), Hoffmann (I. c.), and Thiele (27) shows that little of diagnostic value can be learned from a study of the urinary pepsin. Wilen- ko has shown Viagra Buy Generic that the pepsin output is independent of any special food and that in diabetes Viagra Buy Generic the amount is not always increased. He suggests that when there is a deficiency of secreting surface in the stomach, that pepsin in urine will he diminished and . Viagra Buy Generic 78o GOODMAN: PEPSIN AND DIASTASE IN URINE. [New York Medical Jourx>